





| 货号 | 包装规格 | 操作 |
|---|---|---|
| SAB5700594-100UG | 100 μg |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Recombinant SARS-COV-2(2019-nCoV) Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val16-Arg685) of SARS-COV-2(2019-nCoV) Spike S1 (Accession #YP_009724390.1) fused with a 6XHis tag at the C-terminus.
通过功能性ELISA测定其结合能力。2μg/mL(100 μL/孔)的固定化重组SARS-COV-2 Spike S1可与重组人ACE 2结合,线性范围为0.5-8.7 ng/mL。|COOH芯片上的固定化人ACE 2可以结合SARS-COV-2 Spike S1,SPR测定的亲和力常数为11.4 nM。
对于冻干蛋白:在无菌蒸馏水中复溶至0.1-0.5 mg/mL的浓度。
冻干蛋白质在-20 ° C至-80 ° C下长期储存。复溶后,蛋白质溶液在-20 ° C下可稳定储存3个月,在2-8 °C下可稳定储存1周。本品在≤ -70 ° C下自接收之日起可稳定储存6个月。为获得最佳储存条件,离心后分装成少量,并在推荐温度下储存。
| 英文名称 | Recombinant 2019-nCoV Spike S1 Protein with His tag |
| NACRES | NA.26 |
| UNSPSC | 12352203 |
| 重组 | 在HEK 293细胞中表达 |
| 含量测定 | >90%(SDS-PAGE); >95%(HPLC) |
| 形态 | 冻干粉 |
| 分子量 | 75.8 kDa |
| 杂质 | <1.0 EU/μg蛋白质(LAL试验) |
| UniProt 登录号 | P0DTC2 |
| 运输状态 | 湿冰 |
| 储存温度 | −20°C |
| 质量等级 | 100 |
| 基因信息 | SARS virus ... S(43740568) |
| 包装规格(货号 SAB5700594-100UG) | 100 μg |