| 货号 | 包装规格 | 操作 |
|---|---|---|
| ABE1791-25UL | 25 μL | |
| ABE1791-100UL | 100 μL |
Histone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone 3.1 is also shown to undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. It has been reported that Histone H3 can also undergo dopaminylation ((H3Q5dop), which can potentiate the binding of adjacent histone post-translational modification interacting proteins and play a vital role in dopaminergic neuronal transcription. This rabbit polyclonal antibody specifically binds to Histone H3 dopaminylated on glutamine 5. (Ref.: Lepack, AE., et al (2020). Science. 368(6487); 197-201).
KLH缀合的线性肽，对应于在谷氨酰胺5处酰化的人组蛋白H3.1 N末端的10个氨基酸。
观察到的目标分子量约为16 kDa。计算值为15.508 kDa。在一些裂解物中可能观察到未表征的条带。
表位：N-末端
Anti-Histone H3Q5ser是一种兔多克隆抗体，可检测谷氨酰胺5乙酰化的组蛋白H3。它靶向距离N-末端10个氨基酸内的表位。
在含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05%叠氮化钠的缓冲液中的纯化兔多克隆抗体。
建议储存温度：+2 ° C至+8 ° C。
Evaluated by dot blot with unmodified and Histone H3Q5-serotonyl peptides.
Dot Blot Analysis: A 1:2,000 dilution from a representative lot detected Histone H3Q5-serotonyl peptide but not the unmodified Histone H3 control peptide (aa 1-10).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
人类老鼠
| 英文名称 | Anti-Histone H3Q5ser |
| UNSPSC | 12352203 |
| NACRES | NA.41 |
| ECL@SS | 32160702 |
| 抗体形式 | 纯化抗体 |
| 克隆 | 多克隆 |
| 形态 | 液体 |
| 净化方式 | 亲和层析 |
| 物种反应性 | 兔、小鼠、人 |
| 集中 | （请参阅批次特定说明。） |
| 技术 | ChIP：适用;斑点印迹：适用;蛋白质印迹：适用 |
| 同种型 | IgG |
| UniProt 登录号 | Q16695 |
| 包装规格（货号 ABE1791-25UL） | 25 μL |
| 包装规格（货号 ABE1791-100UL） | 100 μL |
