Sigma-Aldrich Tamm-Horsfall Glycoprotein

产品概述

Research area: Immuno and CKS



Tamm-Horsfall glycoprotein (THGP) also known as uromodulin is abundantly present in mammalian urine. It is highly expressed in the thick ascending limb of the Henle′s loop in the kidney.PMID 15650774 This protein consists of three epidermal growth factor (EGF) domains and a zona pellucida-like domain. THGP is a member of the glycosylphosphatidylinositol (GPI) protein family and is attached to the cell membrane by a C-terminal GPI- anchor.PMID 21081491 The THGP gene is located on the human chromosome at 16p12.3.PMID 23826568

应用领域

Tamm-Horsfall糖蛋白已被用于永生化近端肾小管细胞和人原代近端肾小管上皮细胞的荧光素酶活性测定。PMID 31879271它还被用作尿液样品中尿调节素的校准标准。PMID 28605509

生化/生理作用

Tamm-Horsfall glycoprotein (THGP) may play a role in antibacterial host defense, inflammatory kidney disease, and the urogenital tract.PMID 15650774 It exhibits protective effects against kidney stone formation by reducing the calcium oxalate precipitation. Mutations in the THGP gene lead to familial juvenile hyperuricemic nephropathy (FJHN), glomerulocystic kidney disease (GCKD), and medullary cystic kidney disease type 2 (MCKD-2).PMID 21081491Tamm-Horsfall Protein (THP) mediates the formation of mucous coat of the bladder, thereby regulating the permeability of the ascending loop of Henle.PMID 21081491 THP is known to adhere to Escherichia coli strains with type 131 and type S32 fimbriae, thereby blocking their interaction with the uroepithelium. Additionally, THP activates cells by directly signaling through Toll-like receptor 4 (TLR4). It has been demonstrated that THP stimulates the production of tumor necrosis factor-alpha (TNF-α) and tissue factor in monocytes through TLR4.

物理形态

用含0.02%叠氮化钠的去离子水冻干。

制备/使用说明

自收到之日起，在+2-8 ℃下储存3个月。请勿冷冻。

技术规格