Sigma-Aldrich PKA, Catalytic Subunit, Bovine Heart

产品概述

研究领域：蛋白激酶A（Protein Kinase A，PKA）是一种丝氨酸蛋白激酶，主要作用于丝氨酸和苏氨酸残基。

生化/生理作用

Protein kinases are crucial for integrating signaling networks within eukaryotic cells.PMID 15023366 It plays an essential role in regulating proliferation and fate determination by influencing Hedgehog signaling transduction. Upon binding of cAMP to the PKA regulatory subunits, the catalytic subunit of PKA dissociates from the PKA holoenzyme and proceeds to phosphorylate target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit is capable of activation without the need for cAMP and is well-suited for in vitro studies of PKA phosphorylation with purified substrates or when introduced into intact cells or cell extracts.

包装信息

批次特定浓度请参见小瓶标签。

物理形态

溶于350 mM磷酸氢二钾、100 µM二硫代葡萄糖，pH值6.8。

制备/使用说明

初始解冻后，等分试样并冷冻（-70 ℃）。

其他说明

Corbin，J.D.等，1988。酶法159，74。Rannels，S.R.等，1983。酶法99，55。Witt，J. J.和Roskoski，R. Jr.，1975。分析生物化学66，253。

一个单位定义为在30°C下每分钟将1 pmol磷酸盐掺入Kemptide底物（目录号05-23-4900）所需的酶量。

技术规格