Sigma-Aldrich Photo-reactive Clickable cis-Sterol Probe

应用领域

用于生物正交标记和富集甾醇结合生物分子的光活化甾醇探针。该甾醇探针含有完整的甾体核心结构，带有一个可紫外激活的二氮丙环交联基团，以及一个用于点击化学介导的报告和亲和标记加合的炔基。该探针是细胞可渗透的，允许在活细胞中原位标记生物分子，PMID 23396283以及体外细胞提取物中。

其他说明

Handle the probes in amber vessels under dim ambient light whenever possible. Aqueous solutions of probes complexed to methyl-β-cyclodextrin (20:1 = m-β-CD:probe molar ratio, 5-20 μM probe) permeate cultured cells in complete serum-containing medium within 30 minutes at 37°C and are cross-linked using near-UV (365 nm) light for 3-10 minutes. Cells can then be harvested and lysed, fractionated or otherwise processed. Click chemistry (Cu(I)-catalyzed 1,3-dipolar cycloaddition of the alkyne with an azide reporter tag such as a fluorophore or biotin, products 760765 or 762024) can be performed in protein samples in PBS for gel-visualization of probe-labeling, MS-based proteomics analysis of probe-mediated affinity enrichments or western blot analysis.PMID 23396283

技术规格