西格玛 Sigma-Aldrich ChemiSCREEN GPR14 Membrane Preparation

产品概述

Urotensin II, a cyclic 11-13 residue peptide expressed in motoneurons of the spinal cord, acts as a potent vasoconstrictor (Coulouarn et al., 1998; Ames et al., 1999). The effects of urotensin II are mediated by binding to a GPCR, GPR14, which is expressed in endothelium, smooth muscle, heart and pancreas (Ames et al., 1999). Genetic deletion of GPR14 in mice renders aortae refractile to the contractile activity of urotensin II without changing baseline hemodynamics (Behm et al., 2003). Pharmacological inhibition of urotensin II/GPR14 interactions prevents renal insufficiency following renal artery ligation (Clozel et al., 2004). Thus, GPR14 is an attractive target for a number of cardiovascular diseases. Chemicon′s GPR14 Membrane Preparations are ideal tools for screening for antagonists of urotensin II/GPR14 interactions. The membrane preparations exhibit a Kd of 0.4 nM for [125I]-urotensin II. With 10 mg/well GPR14 Membrane Prep and 0.3 nM [125I]-urotensin II, a greater than 20-fold signal-to-background ratio is obtained.

转染：全长人GPR 14 cDNA

应用领域

用于放射性配体结合试验的人UT /UT 1/GPR 14 GPCR膜制备。

放射性配体结合测定。

生化/生理作用

GPCR Class：A蛋白靶标：UT/UT 1/GPR 14靶标亚家族：尾加压素II

产品特点

将膜与放射性配体和未标记的竞争剂混合在过滤之前，将GF/C 96孔滤板用0.33%聚乙烯亚胺包被30分钟，然后用50 mM HEPES（pH 7.4）洗涤，0.5%BSA。将结合反应转移到滤板上，并用洗涤缓冲液洗涤3次（每次洗涤每孔1 mL）。将板干燥并计数。

物理形态

将膜在50 mM Tris pH 7.4、10%甘油和1%BSA中调节至0.5 mg蛋白质/ml，快速冷冻，并储存在-80 ° C。一个小瓶通常含有2000个单位，其中一个单位是将产生大于1000 cpm特异性的膜的量。

制备/使用说明

在-70 ° C下冷冻保存长达2年。不要冷冻和解冻。

技术规格