Sigma-Aldrich Inorganic Pyrophosphatase from Escherichia coli，≥100单位/mL，缓冲水溶液

产品概述

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate hydrolysis. It plays an important role in energy metabolism by providing a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis. Nucleic acid synthesis would be energetically impossible in vivo if not coupled with the hydrolysis of pyrophosphate (PPi)PMID 6135978PMID 1323891.

应用领域

This product is based on the native pyrophosphatase from E. coli, Uniprot No. P0A7A9. Pyrophosphatase in E. coli is a homohexameric protein containing 175 amino acids residues per subunitPMID 2848015. This product is a recombinant protein expressed in E. coli and induced by IPTG. Each subunit has a MW of 19.7 kDa and theoretical pI of ~5. The protein activity is Mg2+ dependent and it is a relatively thermostable proteinPMID 8664254.

生化/生理作用

无机焦磷酸酶（PPase）是一种普遍存在的酶，它催化焦磷酸盐转化为磷酸盐。PMID 26296329它在能量代谢中起着重要作用，因为它为生物合成反应（如蛋白质、RNA和DNA合成）提供了热力学拉力。PMID 17981157

产品特点

该产物的纯度最小为95%（SEC-HPLC），活性最小为100单位/mL，可在转录过程中提高RNA产量。

物理形态

本品以含有20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA和50%甘油的水溶液形式提供，在25 °C下滴定至pH 8。

其他说明

在pH 9和25 °C条件下，一个装置每分钟将释放1.0 µ mol无机正磷酸盐。用于测定酶活性的反应缓冲液含有50 mM Tris-HCl，pH 9.0。

技术规格