Sigma-Aldrich Carbonic Anhydrase Isozyme II 人，≥80%，粉末，≥3,000 W-A单位/mg蛋白质

产品概述

≥ 80%，粉末，≥ 3，000 W-A单位/mg蛋白质

应用领域

Human carbonic anhydrase isozyme II has been used to assess its gene fusion abilities for efficient expression and recovery of recombinant proteins. Human carbonic anhydrase isozyme II has also been used to investigate a new process for the synthesis of difluoromethanesulfonamides. Furthermore, CA II from Sigma has been used as a standard to measure the CA activity in lung tissue homogenates. The study analysed the possible relationship between the expression of carbonic anhydrase and non-small cell lung cancer.PMID 23648463 The product has also been used with Freund′s complete adjuvant at 1:1 ratio for antibody production. This study evaluated the quantitative and functional alterations of cytosolic CA isoenzymes in the erythrocytes of glucose-6-phosphate dehydrogenase (G6PD)-deficient individuals.PMID 11718695 The enzyme has also been used in the study of natural phenolic inhibitors of CA II.

生化/生理作用

碳酸酐酶是一种锌金属酶，催化二氧化碳水化为碳酸，参与pH和CO2稳态、碳酸氢盐和CO2转运、生物合成反应、骨吸收、钙化、致瘤等病理生理过程，因此这种酶是可用于治疗各种病理状况如青光眼的抑制剂的重要靶点，CAII分子量约为30 kDa，主要存在于II型肺泡上皮细胞中，由于其独特的定位，推测CAII参与肺功能，如调节液体分泌和CO2排出。PMID 23648463磺胺类、氨基磺酸盐和磺酰胺类是CA的有效抑制剂。

其他说明

一个Wilbur-Anderson（W-A）单位将导致0.02 M Trizma缓冲液的pH值在0 ℃下每分钟从8.3降至6.3（一个W-A单位基本上相当于一个Roughton-Booth单位）。

技术规格