Sigma-Aldrich Carbonic Anhydrase II bovine，≥90%（SDS-PAGE），重组体，在大肠杆菌中表达，≥5,000单位/mg蛋白，缓冲水溶液

产品概述

≥ 90%（SDS-PAGE），重组体，在大肠杆菌中表达，≥ 5，000单位/mg蛋白，缓冲水溶液

应用领域

牛碳酸酐酶II已用作魏氏海链藻碳酸酐酶1的天然凝胶电泳和X射线吸收光谱表征中的对照。

Carbonic anhydrase is used to create carbon dioxide capture systems and to research various purification techniques PMID 22513339. Carbonic anhydrase is also used to study acid-base regulation in fish and carbonic anhydrase type II deficiency syndrome PMID 19448075PMID 22120147. Product C7749, Bovine carbonic anhydrase II (CA II), has been widely used as a model protein in the investigation of the protein folding process.

生化/生理作用

Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications PMID 22120147. Carbonic anhydrases are metalloenzymes since most active sites contain zinc. Carbonic anhydrases are widely distributed in plant and animal tissues where they are involved in diverse physiological processes, such as photosynthesis, pH homeostasis, calcification, and bone resorption. The enzymes can be localized in the cytosol or mitochondria, be membrane bound with extracellular domains, or be secreted. Bovine carbonic anhydrase II (CA II) is a cytosolic, single-chain, approximately 29 kDa enzyme.

物理形态

以含150 mM NaCl的20 mM Tris（pH 7.6）溶液形式提供。

其他说明

一个单位将在0 ℃下每分钟将20 mM Tris缓冲液的pH值从pH 8.3降低至6.3。

技术规格