Sigma-Aldrich Photo-reactive Clickable trans-Sterol Probe，百分之九十五

产品概述

95%

应用领域

这是一种用于生物正交标记和富集甾醇结合生物分子的可光活化甾醇探针。这种甾醇探针含有完整的甾醇核心结构，该结构带有可紫外激活的二氮丙环交联基团以及用于点击化学介导的报告和亲和标记附着的炔基。该探针是细胞-可渗透的，允许在活细胞PMID 23396283以及体外细胞提取物中原位标记生物分子。

其他说明

Handle the probes in amber vessels under dim ambient light whenever possible. Aqueous solutions of probes complexed to methyl-β-cyclodextrin (20:1 m-β-CD:probe molar ratio, 5-20 μM probe) permeate cultured cells in complete serum-containing medium within 30 minutes at 37°C, and are cross-linked using near-UV (365 nm) light for 3-10 minutes. Cells can then be harvested and lysed, fractionated, or otherwise processed. Click chemistry (Cu(I)-catalyzed 1, 3-dipolar cycloaddition of the alkyne with an azide reporter tag such as a fluorophore or biotin, products #760765 or #762024) can be performed in protein samples in PBS for gel-visualization of probe-labeling, MS-based proteomics analysis of probe-mediated affinity enrichments or western blot analysis.PMID 23396283

技术规格