Sigma-Aldrich Calmodulin, Bovine Brain, High Purity，钙调素是一种普遍存在的钙离子结合蛋白，作为一个生理效应器的广泛的生物过程。

产品概述

Research area: Cell Signaling

Calmodulin (CaM) ranks among the most highly conserved protein sequences, with only histone proteins H4 and H3, actin B, and ubiquitin showing greater evolutionary conservation. CaM is a primarily helical protein with N- and C-terminal domains, each containing two paired Ca2+-binding EF-hand motifs. These motifs, with pentagonal-bipyramidal geometry, feature a conserved 12-amino acid sequence (positions 1–12) within a canonical helix-loop-helix structure.PMID 33396740

应用领域

高纯度牛脑钙调素已用于研究褪黑激素-钙调素相互作用PMID 35269623，并通过体外孵育激活钙-钙调素（CaM）依赖性蛋白激酶IIa（CaMK II）a PMID 34302798

生化/生理作用

Calmodulin (CaM) is a ubiquitous Ca²⁺-binding protein distributed in eukaryotic cells that serves as a major physiological effector for a wide range of cellular responses. The Ca²⁺-calmodulin complex is involved in the maintenance of intracellular Ca²⁺ homeostasis, as well as in the regulation of the intracellular concentration of other second messengers, such as cyclic AMP and inositol trisphosphate. Mutations in the CaM sequence can result in diverse and potentially life-threatening pathologies such as ventricular tachycardia, congenital arrhythmia, and long QT syndrome. These mutations have the capacity to modify CaM′s ability to bind to Ca²⁺ and its target proteins and peptides, thereby disrupting their subsequent activity. Ca²⁺/CaM complex and CaM-binding domains (CaMBDs) interactions influence memory formation, muscle contraction, cellular metabolism, and cytoskeletal rearrangements.PMID 33396740

包装信息

是的

物理形态

从30 mM HEPES、1 mM CaCl 2、0.1 mM DTT（pH 7.4）中冻干。

制备/使用说明

复溶后，等分并冷冻（-20 ° C或-70 ° C）。水性储备溶液在-20 ° C下可稳定长达2个月或在-70 ° C下可稳定长达4个月。

批次特定复溶体积请参见小瓶标签。

其他说明

一个单位被定义为引起标准水平的活化剂缺陷型钙调磷酸酶的最大酶活化的50%的钙调蛋白的量。

本产品不得用于动物治疗、体内研究或任何其他与牲畜接触的程序。

技术规格