Sigma-Aldrich ChemiSCREEN CCR1 Membrane Preparation，用于放射性配体结合测定的人CCR 1 GPCR膜制备。

产品概述

CCR1 is a GPCR that binds to a variety of CC ligands, including MIP-1a, RANTES, MCP-3, HCC-1, HCC-2, HCC-4, and MPIF-1 (Olson and Ley, 2002). Lymphocytes, macrophages, dendritic cells, and GM-CSF-activated neutrophils express CCR1 (Kaufmann et al., 2001; Cheng et al., 2001). Two selective, non-peptide small molecule antagonists of CCR1, BX-471 and CP-481,715, have been synthesized (Gladue et al., 2003; Liang et al., 2000). Pharmacological and genetic targeting of CCR1, either alone or in combination with cyclosporin A, reduces cardiac and renal allograft rejection (Gao et al., 2000; Horuk et al., 2001a; Horuk et al., 2001b), allergic encephalomyelitis (Liang et al., 2000), and renal fibrosis (Anders et al., 2002) in experimental models. Chemicon′s CCR1 Membrane Preparations are ideal for screening molecules that disrupt interaction between CCR1 and its ligands. The CCR1 Membrane Preps bind to MIP-1a with a Kd of 1.8 nM, and yield greater than 10-fold signal to background ratio with a MIP-1a concentration of 0.1 nM.

人CCR1全长cDNA

应用领域

放射性配体结合测定。

生化/生理作用

GPCR Class：A

蛋白质靶标：CCR 1

目标亚家族：趋化因子

物理形态

包装缓冲液中的液体：50 mM Tris pH 7.4，10%甘油和1% BSA，不含防腐剂。将膜蛋白在包装缓冲液中调节至指定浓度，快速冷冻，并储存在-80 °C下。每个小瓶通常含有500个单位，其中，1个单位是产生125 I标记MIP-1 α（0.1 nM输入浓度）的特异性结合大于1000 cpm的膜制备量。

制备/使用说明

在-70 ° C下冷冻保存长达2年。不要冷冻和解冻。

分析说明

质量标准：（125 I）MIP-1 α结合的Bmax：3.3 pmol/mg蛋白（125 I）MIP-1 α结合的Kd：约1.8 nM宿主细胞：

技术规格