西格玛 Sigma-Aldrich ChemiSCREEN Membrane Preparation Recombinant Human CXCR7 Chemokine Receptor，用于放射性配体结合试验和GTPγS结合的人CXCR 7/RDC 1 GPCR膜制备。

产品概述

：编码CXCR 7的全长人CXCR 7 cDNA

The orphan receptor RDC1 is phylogenetically related to chemokine receptors, and its gene (CMKOR1, chemokine orphan receptor 1) occupies a chromosomal location near the genes for other CXC receptors. Several immune system cells, including T lymphocytes, mature dendritic cells and memory B cells, express RDC1 (Infantino et al., 2006). A subset of HIV and SIV strains use RDC1 as a coreceptor (Shimizu et al., 2000). The chemokine SDF-1/CXCL12, which binds to CXCR4, has been shown also to bind and activate RDC1 to promote chemotaxis of T lymphocytes; thus, CXCR7 has been proposed to be the new name for RDC1 (Balabanian et al., 2005). I-TAC, one of the chemokines thought to bind to CXCR3 only, has been reported to distinguish CXCR7 from CXCR4 in radioligand binding experiments (Burns et al. 2006). Chemicon′s CXCR7 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at CXCR7. The membrane preparations exhibit a Kd of 0.32 nM for [¹²⁵I]-SDF-1α. With 0.25 nM [125I]-SDF-1α, 10µg/well CXCR7 Membrane Prep typically yields greater than 4-fold signal-to-background ratio.

应用领域

放射性配体结合试验和GTP γ S结合

生化/生理作用

GPCR Class：A

蛋白质靶点：CXCR 7/RDC 1

目标亚家族：趋化因子

产品特点

孵育条件将膜与放射性配体和未标记的竞争剂混合在过滤之前，将GF/C 96孔滤板用0.33%聚乙烯亚胺包被30分钟，然后用50 mM HEPES（pH 7.4）洗涤，将结合反应转移至滤板，并洗涤3次结合缓冲液：50 mM Hepes，pH 7.4，5 mM MgCl 2，1 mM CaCl 2，0.2% BSA，过滤并在4 ℃下储存。（125 I）SDF-1α（Perkin Elmer#：NEX-346）洗涤缓冲液：50 mM Hepes，pH 7.4，500 mM NaCl，0.1% BSA，过滤并储存在4 ℃。一个包装含有足够用于至少200次测定（单位）的膜，其中一个单位是将产生大于4倍信号的膜量：0.25 nM 125 I标记的SDF-1α背景

物理形态

包装缓冲液中的液体：50 mM Tris pH 7.4，10%甘油和1% BSA，不含防腐剂。包装方法：用包装缓冲液将膜蛋白调节至1 mg/mL，分装1 mL/瓶。小瓶快速冷冻，-80 °C保存。

制备/使用说明

在-70 ° C下冷冻保存长达2年。不要冷冻和解冻。

分析说明

表1.信号：在使用CXCR 7膜制备物和未标记的SDF-1α的竞争结合试验中获得的背景和特异性结合值。10 µg/孔信号：背景4.92特异性结合（cpm）15919 1单位= 10 µg（125 I）-SDF-1 α结合的Bmax：0.51 pmol/mg蛋白质（125 I）- SDF-1α结合的Kd：~0.32 nM

技术规格