| 货号 | 包装规格 | 操作 |
|---|---|---|
| SRP0255-100UG | 100 μg |
EIF4E (eukaryotic translation initiation factor 4E) is a m7GpppN (N is any nucleotide) cap-dependent RNA-binding protein.PMID 25615552 It is one of the three subunits of the translation initiation factor complex eIF4F, and is the least common of all initiation factors.
MNK1激酶的合适底物。
EIF4E (eukaryotic translation initiation factor 4E) is a part of eIF4F complex, which is responsible for the recruitment of ribosomes to the capped end of mRNAs during mRNA translation. It acts as the rate-limiting element in cap-dependent mRNA translation in eukaryotic cells. It promotes the translation of pro-oncogenic mRNAs, including vascular endothelial growth factor (VEGF), cyclin D3, and Mcl-1 (induced myeloid leukemia cell differentiation). Up-regulation of this protein is linked with various cancers, such as prostate cancer, breast cancer, and lymphoma. Studies show that this protein is overexpressed in melanoma cell lines and is linked with resistance to vemurafenib. Thus, it might have potential as a therapeutic target to suppress melanoma cell proliferation and abrogating vemurafenib resistance.PMID 25615552
在50 mM Tris-HCl（pH 7.5）、150 mM NaCl、0.25 mM DTT、0.1 mM EGTA、0.1 mM EDTA、0.1 mM PMSF、50%甘油中配制。
在冰上解冻。第一次解冻时，短暂旋转含酶的试管，以回收试管中的全部内容物。将酶等分为一次性使用的等分试样。将剩余的未稀释的酶等分试样储存在-70 °C下。注：酶对冻融循环非常敏感。
| 英文名称 | eIF4E Active human |
| NACRES | NA.32 |
| UNSPSC | 12352200 |
| 生物来源 | 人类 |
| 重组 | 在大肠杆菌中表达 |
| 含量测定 | ≥70%（SDS-PAGE） |
| 形态 | 水溶液 |
| 分子量 | 51 kDa |
| 包装 | 100 μg包装 |
| 集中 | >0.02 mg/mL |
| 技术 | 基于细胞的试验：适用 |
| 适用性 | 适用于分子生物学 |
| NCBI 登录号 | NM_001968 |
| UniProt 登录号 | P06730 |
| 运输状态 | 干冰 |
| 储存温度 | −70°C |
| 基因信息 | 人类... EIF4E（1977） |
| 包装规格（货号 SRP0255-100UG） | 100 μg |
