Sigma-Aldrich EPHA3 (571-end), active, GST tagged human，PRECISIO®激酶，重组，在杆状病毒感染的Sf 9细胞中表达，≥70%（SDS-PAGE），甘油缓冲水溶液

产品概述

EPHA 3是一种受体酪氨酸激酶（RTK），属于RTK中最大的亚类，称为ephrin受体组。它从胎盘阶段到整个发育过程都有高水平的表达。PMID 19678838它是一种I型跨膜蛋白，有一个膜结合的配体。它既是一个癌基因，也是一个肿瘤抑制基因。PMID 23324396 PMID 24940039这种蛋白存在于细胞质和质膜中。PMID 23970317

生化/生理作用

EPHA3 binds the ephrin-A ligand and has diverse cellular function. Analysis of human colorectal, breast, lung and pancreatic cancer samples shows somatic mutations in the EPHA3 gene.PMID 16941478 EPHA3 gene expression can be regulated by CD28 and IGF-1 in Jurkat cells and expression of EPHA3 is associated with adherence and motility of malignant T cells.PMID 14697337 It maintains the less differentiated state of cancer cells, by activating MAPK (mitogen-activated protein kinase) pathway. It is over-expressed in glioblastoma (GBM) cells, especially during the initial stages. It also has higher expression levels in aggressive mesenchymal subtype of GBM.PMID 23410976 In hepatocellular carcinoma (HCC), it predicts increased invasiveness and poor patient prognosis.PMID 23970317

物理形态

以50 mM Tris-HCl（pH 7.5）、150 mM NaCl、0.25 mM DTT、0.1 mM EGTA、0.1 mM EDTA、0.1 mM PMSF和25%甘油供应。

技术规格