Sigma-Aldrich ERK1，活性，未标记 人，PRECISIO®激酶，重组，在杆状病毒感染的Sf 9细胞中表达，≥70%（SDS-PAGE），甘油缓冲水溶液

产品概述

细胞外信号调节激酶1（extracellular signal-regulated kinase 1，ERK 1）属于丝裂原活化蛋白激酶（mitogen-activated protein kinases，MAPK）/ERK信号通路中的中间蛋白家族。该基因含有9个外显子和8个内含子，全长9 kb。它是一种组成型表达蛋白，其活性随磷酸化状态而改变。在神经组织中表达量较高。它含有11个激酶亚结构域，PMID 9628824它的分子量为44 kDa。PMID 15509801

生化/生理作用

Extracellular signal-regulated kinase 1 (ERK1) act as intermediate in multiple signaling cascades, and is acted upon by MEK protein. MEK are essential for Golgi fragmentation during mitosis, and this is achieved by its action upon an alternatively spliced isoform of ERK1, called ERK1c.PMID 16533948 ERK1/2 signaling plays a key role in cellular response to topoisomerase II poisoning, by activating G2/M checkpoint, and thus, prevents poison-induced cell death.PMID 23166842 In melanocytes, pleiotrophin suppresses pigmentation or melanogenesis, by degradation of MITF, through the activation of ERK1/2 signaling.PMID 25141921

ERK 1（MAPK 3）参与细胞信号级联反应，其响应于许多生长因子和细胞因子而被激活。MAP激酶信号传导功能的破坏与包括炎症、癌症和神经退行性疾病在内的疾病状态相关。

物理形态

以25 mM MOPS（pH 7.5）、300 mM NaCl、0.25 mM DTT、0.1 mM EDTA、0.1 mM PMSF和25%甘油供应

技术规格