Sigma-Aldrich Rhodamine labeled D-Lysine，适用于荧光微生物成像

产品概述

Bacterial Peptidoglycan is a polymer consisting of sugars and amino acids, a mesh-like rigid layer that forms the cell wall. D-Amino Acids (D-AAs) are metabolically incorporated onto the bacterial cell wall by D,D-transpeptidase and/or L,D-transpeptidase. It was recently discovered that those transpeptidases catalyze the metabolic incorporation of exogenous D-AAs with almost no restriction of the side-chain identity.PMID 21792174 Modification of a D-AA with molecular fluorophores provides fluorescent D-amino acids that can efficiently label in situ peptidoglycan in diverse bacterial species.PMID 23055266 Rhodamine-Labeled D-lysine (TDL) is a fluorescent derivative of D-lysine that is obtained by a synthetic conjugation of D-lysine to the fluorophore. Rhodamine Labeled D-lysine has high biocompatibility and suitability for labeling peptidoglycans in live bacteria. Additionally, it can be used in tandem with other stains such as FITC-Labeled D-Alanine (SBR00049) to distinguish between different bacteria. Other compatible products useful for live staining include: Rhodamine B Labeled Polymyxin B, FITC Labeled D-Lysine, FITC Labeled Vancomycin and Dansyl Labeled Polymyxin B (SBR00036, SBR00050, SBR00028, SBR00029 and SBR00047).

应用领域

荧光标记的D-氨基酸（FDAA）可用于许多应用，包括：PMID 28989665 PMID 28900018细菌细胞壁形态细菌细胞壁形成或重塑活性细菌活力/活性鉴定表面或物质中的细菌活性根据不同D氨基酸和糖的掺入谱区分各种细菌菌株PMID 33209242

分析说明

荧光显微镜应用：罗丹明标记的D-赖氨酸的激发/发射波长范围为550/570 nm。在荧光显微镜成像应用中，推荐的工作浓度为工作介质中的250 µM-500 µM。DMSO溶液的等分试样可在-20 °C下避光储存至少一个月。

技术规格