密理博 默克密理博 n-Dodecyl β-D-maltoside，ULTROL®级，≥98%（HPLC），非离子型清洁剂，用于酶的稳定和活化

产品概述

A water-soluble nonionic detergent. Studies on rhodopsin have shown that alkyl glycosides with longer alkyl chains are better at preserving the protein′s properties. In addition, dodecyl maltoside′s longer hydrophilic group confers higher solubility in water. This is in contrast to undecyl and dodecyl glucosides, which have low solubility in water. Due to its low CMC, dodecyl maltoside is not easily removed by dialysis. The half time for dialysis is 20 h. This detergent is milder than nonyl glucoside. Of several glycosides tested, dodecyl maltoside was found to be the most useful for the preparation of active cytochrome oxidase from Neurospora. In octyl glucoside, cytochrome oxidase dissociated into its subunits and became inactive. Similarly, beef heart oxidase when equilibrated in octyl glucoside formed a mixture of polymer, dimer, and lower molecular weight forms and underwent denaturation. In contrast, the enzyme, when equilibrated in dodecyl maltoside, was found to exist as a uniform species of 30 kDa, which is consistent with an active dimeric form. Dodecyl maltoside has a critical micelle concentration (CMC) of 100-600 µM and a micellar weight of 70,000. Aggregation number: 98.

吸光度（10%，H2O，260 nm）：<1.0。

非离子去污剂，类似于正辛基-β-D-吡喃葡萄糖苷;然而，增加烷基链长度已被证明可增加溶液中无脂质视紫红质的热稳定性。可用于膜蛋白溶解研究。聚集数：98。

应用领域

使用质谱兼容表面活性剂在蛋白质组学和抗体开发中最大化疏水肽回收。本文探讨了在蛋白质组学研究中使用正十二烷基-β-D-麦芽糖苷来最大化疏水肽的回收，研究结果突出了该表面活性剂与质谱的兼容性，改善了肽分析和抗体开发。（Nie等，2022）.用于在线稀有细胞分离和串联无标记定量蛋白质组学的镰刀状惯性微流体系统（Orcs-Proteomics）.本研究介绍了一种利用正十二烷基β-D-半乳糖基的惯性微流控系统，麦芽糖苷在稀有细胞分离和蛋白质组学研究中的应用，该研究强调了表面活性剂在促进细胞回收和蛋白质组学分析中的作用。（Wang等人，2022）。用于无标记单细胞蛋白质组学的表面活性剂辅助一锅法样品制备。：本文介绍了一种使用正十二烷基β-D-麦芽糖苷进行单细胞蛋白质组学的方法。表面活性剂有助于有效的样品制备，促进高分辨率蛋白质组学分析。（Tsai等人，2021）。

制备/使用说明

复溶后，将其冷却（4 ° C）。贮备液在4 ° C下可稳定储存长达3个月。

其他说明

Witzmann，F.，et al. 1991. Electrophoresis 12，687. Bass，W.T.，and Bricker，T.M. 1988. Anal. Biochem. 171，330. Rozarar，P.，et al. 1980. Biochemistry 19，4108. Grip，W.J.，and Bovee-Geurts，P.H.M. 1979. Chem. Phys. Lipids 23，321. Stubbs，G.W.，等人，1976. Biochim. Biophys. Acta 425，46.

技术规格