| 货号 | 包装规格 | 操作 |
|---|---|---|
| A7189-250UN | 250 units | |
| A7189-500UN | 500 units |
L-丙氨酸脱氢酶具有N-末端底物结合域和C-末端NAD-结合域。
枯草芽孢杆菌L-丙氨酸脱氢酶已被用于碳纳米管柱中进行氢驱动的生物催化氢化研究。
L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection PMID 8999934.
L-丙氨酸脱氢酶是枯草芽孢杆菌芽孢形成所必需的酶。
L-Alanine dehydrogenase is a stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate and NADH are released in that specific order.PMID 8999934 Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.PMID 14298830
悬浮于2.4 M（NH 4）2SO 4溶液中，pH 7.0
在pH 10.0和25 ° C条件下，每分钟一个单位可将1.0 μ mol L-丙氨酸转化为丙酮酸和NH3。
| 英文名称 | L-Alanine Dehydrogenase from Bacillus subtilis |
| CAS 号 | 9029-06-5 |
| UNSPSC | 12352204 |
| NACRES | NA.54 |
| EC 号 | 232-847-9 |
| MDL 号 | MFCD00131441 |
| 生物来源 | 枯草芽孢杆菌 |
| 形态 | 硫酸铵悬浮液 |
| 比活性 | ≥ 20单位/mg蛋白质（Lowry） |
| 储存温度 | 2-8°C |
| 质量等级 | 200 |
| 包装规格（货号 A7189-250UN） | 250 units |
| 包装规格（货号 A7189-500UN） | 500 units |
