| 货号 | 包装规格 | 操作 |
|---|---|---|
| A7419-2ML | 2 mL |
Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissuesPMID 6166661. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Avidin-Peroxidase has been used for ELISA applicationsPMID 12444126PMID 17692276. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.
Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml. PMID 12724354
0.01 M磷酸盐缓冲盐水溶液，pH 7.4，含0.05% MIT。
亲和纯化蛋白。
| 英文名称 | Avidin–Peroxidase |
| UNSPSC | 12352203 |
| NACRES | NA.46 |
| MDL 号 | MFCD00130587 |
| 生物来源 | 从鸡蛋白色中提取的抗生物素蛋白 |
| 偶联物 | 过氧化物酶缀合物 |
| 形态 | 缓冲水溶液 |
| 技术 | indirect ELISA: 1:100,000; western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well) |
| 运输状态 | 干冰 |
| 储存温度 | −20°C |
| 质量等级 | 200 |
| 包装规格（货号 A7419-2ML） | 2 mL |
