色谱科 默克Supelco 4-(二甲氨基)偶氮苯-4′-磺酰氯，衍生级（HPLC），LiChropur™，98.0-102.0%（AT）

产品概述

Amino groups are common to many biological compounds and many amines used in pharmaceuticals. Most of these compounds show weak absorption in direct HPLC analysis with UV-VIS detection. However, strongly absorbing derivatives of amines can be easily detected by HPLC with high sensitivity even when present at low concentrations. Primary and secondary amino groups are usually derivatized as aromatic derivatives by nucleophilic substitution reactions using reagents such as arylsulfonyl chlorides.
4-(Dimethylamino)azobenzene-4′-sulfonyl chloride, a well-known UV-labeling agent, is generally used for the derivatization of N-terminal amino acids, imidazole derivatives, polyamines, etc. prior to their chromatographic determination due to the former′s capacity to covalently bind to the substrates. Its derivatives typically absorb UV radiation between the range of 436-460 nm. This method of derivatization is regarded as superior compared to other methods owing to the simplicity of the procedure, high stability and reproducibility, best resolution of the analytes, etc.

应用领域

4-（二甲氨基）偶氮苯-4 ′-磺酰氯可用于生物胺、伯氨基酸、三聚氰胺、羟脯氨酸和脯氨酸的衍生化，以提高其检测灵敏度。

其他说明

了解适用于HPLC或LC-MS分析的LiChropur ™试剂

技术规格