Sigma-Aldrich 胰蛋白酶原 来源于牛胰腺，基本无盐，冻干粉末，≥10,000 BAEE单位/mg蛋白（E1%/280，活化为胰蛋白酶后）

产品概述

胰蛋白酶原（Trypsinogen）是一种在胰腺内合成并在小肠内被肠激酶激活的酶原，是由胰蛋白酶原的NH2端经6个二硫键连接而成的多肽链。肠激酶在胰蛋白酶原的Lys6-末端裂解一个六肽，使其与胰蛋白酶原的NH2端形成一个六肽连接。胰蛋白酶原是由胰蛋白酶原的NH2端经6个二硫键连接而成的多肽链。胰蛋白酶原是由胰蛋白酶原的NH2端经6个二硫键连接而成的多肽链。胰蛋白酶原是由胰蛋白酶原的NH2端经6个二硫键连接而成的多肽链。Ile7肽键结合并激活它。由此形成的胰蛋白酶自动催化激活更多的胰蛋白酶原为胰蛋白酶。这种天然形式的胰蛋白酶被称为β-胰蛋白酶。其在Lys131-Ser132处发生自溶，产生α-一种通过二硫键连接在一起的胰蛋白酶。胰蛋白酶是一种丝氨酸蛋白酶，其活性部位有组氨酸46和丝氨酸183。胰蛋白酶的最适pH值为7 - 9。PMID 5466615

应用领域

Trypsinogen from bovine pancreas is suitable for use in:
the secondary structure analysis of proteins in H2O solution using single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopyPMID 12199577tuning and calibration of electrospray ionization quadrupole time-of-flight (ESI-Q-TOF) mass spectrometerPMID 15543996the secondary structure analysis of proteins by infrared (IR) spectroscopyPMID 2226461SDS-PAGE as a molecular weight standard (24kDa)PMID 10518779

生化/生理作用

Phytic acid complexed with calcium has been shown to increase the secretion of trypsinogen unable to be cleaved for activation. It also reduced the stabilization effect of calcium on activated trypsin. The active form of trypsinogen, referred to as trypsin, cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. It also hydrolyzes ester and amide linkages of synthetic derivatives of amino acids such as, benzoyl L-arginine ethyl ester (BAEE), p-toluenesulfonyl-L-arginine methyl ester (TAME), etc.PMID 5466615PMID 14224394

其他说明

一个BAEE单位等于以BAEE为底物，在pH 7.6、25 ° C和3.2 mL反应体积（1 cm光程）条件下，Δ A253为0.001/min。

技术规格