Sigma-Aldrich p75 human，重组体，在大肠杆菌中表达，≥70%（SDS-PAGE）

产品概述

p75 was first identified during micro-sequencing of a protein that co-purified with positive cofactor 4 (PC4), a general transcriptional coactivator. This is a transcriptional coactivator and has a molecular weight of 75kDa. This protein is also known as LEDGF (lens epithelium-derived growth factor), and belongs to the hepatoma-derived growth factor (HDGF) family. p75 is constitutively expressed in the nucleus. This gene is localized to human chromosome 9p22.3, and is composed of 15 exons and 14 introns. It is transcribed into two splice variants- p52 and p75 both of which contain 325 residues in their N-terminals. They differ in their C-terminal, where p75 contains 205 residues in its C-terminal. Its C-terminal also contains the 83-residue IN binding domain (IBD) from residues 340–417, which is essential for p75′s virological and cellular characteristics.

应用领域

p75 human has been used in immunohistochemistry.PMID 26428886

生化/生理作用

p75和p52蛋白与染色质紧密结合，并与核内其它蛋白相互作用，这些蛋白质参与转录调控。慢病毒利用这种蛋白质的染色质束缚能力整合到宿主染色体中。p75不仅锚定HIV，人乳头瘤病毒（Human papillomavirus，HPV）E6/E7癌基因在宿主细胞中促进p75的表达，其依次赋予HPV阳性癌细胞抵抗不同类型的细胞应激如DNA损伤的保护。

人p75蛋白与p52蛋白相似，是一种非TAF的转录辅激活子，在体外介导RNA聚合酶Ⅱ介导的激活子依赖性转录。虽然p75和p52蛋白是由同一基因选择性剪接而成的，p75作为透镜上皮细胞衍生的生长因子（LEDGF）参与上皮细胞的生长，作为自身抗原参与特应性皮炎的发病。

人p75蛋白与p52蛋白相似，是一种非TAF的转录辅激活子，在体外介导RNA聚合酶Ⅱ介导的激活子依赖性转录。虽然p75和p52蛋白是由同一基因选择性剪接而成的，p75作为透镜上皮细胞衍生的生长因子（LEDGF）参与上皮细胞的生长，作为自身抗原参与特应性皮炎的发病。

物理形态

澄清无色冷冻液体溶液

制备/使用说明

使用手动冷冻机，避免反复冻融循环。工作时，请将样品置于冰上。

技术规格