| 货号 | 包装规格 | 操作 |
|---|---|---|
| SRP0253-100UG | 100 μg |
The gene E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is mapped to human chromosome 8p11-p12. It belongs to the translation repressor protein family.
Human E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1)(GenBank Accession No. NM_004095), full length with N-terminal His tag, MW = 13.6kDa, expressed in Escherichia coli.
4 EBP 1活性人已被用作体外激酶测定的底物。PMID 26385560
E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is involved in protein synthesis, cell growth and survival. In unphosphorylated form, it suppresses the translation initiation of capped mRNAs. On the other hand, in phosphorylated form, it increases translation initiation. Phosphorylation of E1F4EBP1 releases it from eIF4E (eukaryotic translation initiation factor 4E), thereby resulting in cap-dependent translation. E1F4EBP1 is also involved in tumorigenesis and the gene is overexpressed in various carcinomas. It is also a substrate for mTORC1 (mammalian target of rapamycin complex 1). In non-phosphorylated conditions, it interacts with p21 (cyclin-dependent kinase inhibitor) and enhances destabilization of p21.PMID 26832959
在50 mM Tris-HCl（pH 7.5）、150 mM NaCl、0.25 mM DTT、0.1 mM EGTA、0.1 mM EDTA、0.1 mM PMSF和50%甘油中配制。
在冰上解冻。第一次解冻时，短暂旋转含酶的试管，以回收试管中的全部内容物。将酶等分为一次性使用的等分试样。将剩余的未稀释的酶等分试样储存在-70 °C下。注：酶对冻融循环非常敏感。
| 英文名称 | 4EBP1 Active human |
| UNSPSC | 12352202 |
| NACRES | NA.32 |
| MDL 号 | MFCD01094895 |
| 生物来源 | 人类 |
| 重组 | 在大肠杆菌中表达 |
| 含量测定 | ≥70%（SDS-PAGE） |
| 形态 | 水溶液 |
| 分子量 | 13.6 kDa |
| 包装 | 100 μg包装 |
| 储存条件 | 避免反复冻融 |
| 集中 | >0.02 mg/mL |
| NCBI 登录号 | NM_004095 |
| UniProt 登录号 | Q13541 |
| 运输状态 | 干冰 |
| 储存温度 | −70°C |
| 基因信息 | 人类EIF 4 EBP 1（1978年） |
| 包装规格（货号 SRP0253-100UG） | 100 μg |
