Sigma-Aldrich Biliverdin Reductase A human，重组体，在大肠杆菌中表达，≥90%（SDS-PAGE），缓冲水溶液

产品概述

研究领域：胆绿素还原酶A（BVRA）是胆绿素还原酶的一种同工酶，是一种细胞膜受体，由两个主要的区域组成，即调节/DNA相互作用区和催化区，PMID 25726384

应用领域

Biliverdin Reductase Ahuman has been used as a component of the incubation medium to evaluate theactivity of hemeoxygenase 1.PMID 32924610 It has also been used as a standard todetermine the age of the bleed from haemorrhagic lesion.PMID 36995028

生化/生理作用

Biliverdin reductase catalyzes the transformation of the blue-green pigment biliverdin IX to the yellow-orange bile pigment bilirubin IX by converting a double-bond between the second and third pyrrole ring into a single-bond.PMID 4378982 This enzyme has two distinct cofactor-dependent pH optima. In the acidic range of pH 6.0-6.7, NADH is utilized, whereas in the alkaline range of pH 8.5-8.7, NADPH is utilized.PMID 6256351 Biliverdin reductase is considered a major physiologic cytoprotectant. Biliverdin reductase suppresses experimental autoimmune encephalomyelitis in rats.PMID 16540391 Depletion of biliverdin reductase leads to accumulation of cellular oxidants and augmented cell death.PMID 16461755Biliverdin reductase (BVR)modulates the functions of insulin via the insulin-like growth factor 1 (IGF-1)pathway. It shows dual specificity kinases where it phosphorylates tyrosineapart from serine and threonine. BVR acts as a biomarker in certain cancers.PMID 26089799

物理形态

含有20 mM磷酸钾缓冲液、10%甘油、0.2% Igepal CA 630、1 mM EDTA和0.1 mM DTT的溶液。

其他说明

在pH 8.5、100 mM磷酸钾缓冲液和37 °C条件下，在NADPH依赖性反应中，1单位胆绿素还原酶每分钟可将1纳摩尔胆绿素转化为胆红素。

技术规格