Sigma-Aldrich Cathepsin S Active human，重组体，在辅理善™ 293-F细胞中表达，≥90%（SDS-PAGE）

产品概述

Research area: Cell Signaling

Human cathepsin S (GenBank Accession No. NM_004079.3), CD33 signal peptide (amino acids 1-16) + cathepsin S (amino acids 17-331), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells. Cathepsin S belongs to the cysteine cathepsin protease family. It has limited tissue expression, being associated with antigen-presenting cells localized in lymph and spleen, as well as other immune cells like macrophages. Human cathepsin S is produced from its corresponding CTSS gene on chromosome 1q21 and is synthesized as a pre-proenzyme.PMID 25872877

应用领域

Active human cathepsin S has been used:
to assess the pathogenesis of Alzheimer′s disease. PMID 21585286 to investigate the optimization of selectivity of Azepanone-based inhibitors. PMID 21733692for the immunocytochemical detection of cathepsin-S in mouse samples.PMID 32719104 for incubating mouse brain sections to test the impact of cathepsin-S on perineuronal nets (PNNs) integrity.PMID 32719104

生化/生理作用

组织蛋白酶S作为一种溶酶体蛋白酶，能促进内-溶酶体途径中有害和受损蛋白的降解，并在主要组织相容性复合体（MHC）II类抗原提呈中发挥特殊作用。组织蛋白酶S的调节异常与多种病理状态有关，如癌症、关节炎、PMID 25872877组织蛋白酶S可用于酶动力学研究、筛选抑制剂和选择性分析。它也用作动脉粥样硬化、糖尿病、肥胖和动脉粥样硬化形成的生物标志物。此外，它作为一种有希望的靶点对抗脑穿透和治疗多发性硬化症和神经性疼痛。

物理形态

在45 mM Tris-HCl（pH 8.0）、124 mM NaCl、2.4 mM KCl、225 mM咪唑、3 mM DTT和10%甘油中配制。

制备/使用说明

在冰上解冻。第一次解冻时，短暂旋转含酶的试管，以回收试管中的全部内容物。将酶等分为一次性使用的等分试样。将剩余的未稀释的酶等分试样储存在-70 °C下。注：酶对冻融循环非常敏感。

其他说明

一个单位定义为在37°C下每分钟切割1 pmol底物的酶量

技术规格