Sigma-Aldrich DNMT3A/DNMT3L，活性，人，重组，在杆状病毒感染的Sf 9细胞中表达，≥90%（SDS-PAGE）

产品概述

DNMT3A（DNA（cytosine-5）-methyltransferase 3A）基因定位于人类染色体2p23。编码的蛋白质有2种异构体，DNMT 3A1和DNMT 3A2。DNMT 3A1广泛表达，DNMT 3A2主要存在于胚胎干细胞中（卵巢和睾丸）. DNMT3L（DNA（cytosine-5）-methyltransferase 3-like）属于Dnmt 3家族。它不能使DNA甲基化。该蛋白质有一个富含半胱氨酸的区域，含有一个新的-该基因定位于人类染色体21q22.3。

应用领域

DNMT3A/DNMT3L活性人已用于体外甲基化测定和REMSA（RNA电泳迁移率变动测定）中以研究ecRNA（额外编码RNA）在DNA甲基化中的作用。PMID 27384705

生化/生理作用

DNMT3A (DNA (cytosine-5)-methyltransferase 3A) is a DNA methyltransferase. It is responsible for the methylation of the cytosine residue in 5′-C-phosphate-G-3′ (CpG) dinucleotides. DNMT3A plays a significant role in immunoregulation (adaptive as well as innate immune responses). It is also the most commonly mutated gene in hematologic malignancies. DNMT3A is a commonly mutated gene in acute myeloid leukemia and is responsible for an unfavorable prognosis.
DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) is mainly responsible for the stimulation of DNA methylation. It is a non-catalytic accessory factor. It helps the DNA methylation machinery to be placed on properly chromatinized DNA regions. It also helps the poorly methylated area, thereby providing uniform methylation patterns.PMID 20838592 Mutations in the DNMT3L gene might be associated with azoospermia susceptibility.

技术规格