Sigma-Aldrich MIF human，重组，在昆虫细胞中表达，≥98%（SDS-PAGE），≥98%（HPLC）

产品概述

Macrophage migration inhibitory factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through cluster of differentiation 74 and 44 (CD74 and CD44), resulting in the secretion of tumor necrosis-α (TNF-α), interleukins-1, -6, -8, and various matrix metalloproteinases (MMPs). The gene encoding this protein is localized on human chromosome 22q11.23. Insect cell derived recombinant MIF is a 15kDa protein containing 124 amino acid residues, including an N-terminal His-tag.

生化/生理作用

巨噬细胞移动抑制因子（MIF）的酶活性以其作为一种互变异构酶的能力为特征，能够催化酮基苯丙酮酸和L-多巴色素的酮基异构化为烯醇，似乎MIF的催化活性依赖于其三聚体构型和游离的N-末端脯氨酸残基。

物理形态

从10 mM磷酸钠（pH 7.0）中冻干。

制备/使用说明

打开前将小瓶冲洗干净。用水复溶至浓度为0.1 - 1.0 mg/ml。不要涡旋。该溶液可在2 - 8 ° C下储存长达1周。为了延长储存时间，建议在含有载体蛋白（例如0.1% BSA）的缓冲液中进一步稀释，并在-20 °C至-80 °C下以工作等分试样储存。

技术规格