



| 货号 | 包装规格 | 操作 |
|---|---|---|
| C1235-100UN | 100 units |
重组,冻干粉,≥10单位/mg蛋白
Cholesterol oxidase is used to determine serum cholesterol.PMID 4818200PMID 14687904 The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids.PMID 12082166 Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.PMID 11457962 CHOD has also been used as a molecular probe to elucidate cellular membrane structures.PMID 14730988PMID 6247347
胆固醇氧化酶(Cholesterol oxidase,CHOD)是一种含有FAD的黄素蛋白单体,催化胆固醇分解的第一步。CHOD是一种双功能酶,在需要FAD的步骤中将胆固醇氧化为胆甾-5-烯-3-酮,然后通过释放H2 O2异构化为胆甾-4-烯-3-酮。CHOD的最适pH为7.0。Hg 2+,Ag +,离子去污剂抑制酶活性。
溶于冷的50 mM磷酸钾缓冲液(pH 7.0)中。临用前制备溶液。
在37 °C和pH 7.0下,在过氧化物酶连接系统中,一个单位每分钟将1.0 μmol胆固醇转化为4-胆甾烯-3-酮。
| 英文名称 | Cholesterol Oxidase microbial |
| CAS 号 | 9028-76-6 |
| UNSPSC | 12352204 |
| NACRES | NA.54 |
| EC 号 | 232-842-1 |
| MDL 号 | MFCD00130783 |
| 形态 | 冻干粉 |
| 比活性 | ≥10单位/mg蛋白质 |
| 分子量 | 55 kDa |
| 溶解性 | 50 mM磷酸钾缓冲液,pH 7.0:可溶 |
| 储存温度 | −20°C |
| 质量等级 | 200 |
| 包装规格(货号 C1235-100UN) | 100 units |