Sigma-Aldrich Matrix Metalloproteinase-7 human，~0.1 mg/mL，重组，在大肠杆菌中表达，缓冲水溶液

产品概述

基质金属蛋白酶-7（Matrix metalloproteinase-7，MMP-7）又称基质溶解素（matrilysin），是人类染色体11 q21-q22上的一个基因，是MMP家族中最小的成员，在癌细胞中高度表达，仅由上皮细胞释放。PMID 25875355

应用领域

人基质金属蛋白酶-7（MMP-7）已用于串珠素MMP-7蛋白水解的计算机分析。PMID 24833109

生化/生理作用

Matrix metalloproteinase-7 (MMP-7) catalyzes the cleavage of extracellular matrix (ECM) proteins such as proteoglycans, fibronectin, entactin, laminin, collagen III/ IV/ V/ IX/ X/ XI, type I/ II/ IV/ V gelatins, and elastin.PMID 25875355 Matrix metalloproteinase-7 facilitates initial stages of tumor progression. Activated MMP7 plays a vital role in human colorectal cancer (CRC) liver metastases. Overexpression of MMP7 is observed in various human cancers, such as breast, lung, prostate, esophagus, stomach, endometrium, and ovarian carcinomas, as well as esophageal squamous cell carcinomas. Increased expression of MMP7 is also associated with pathogenesis of demyelinating multiple sclerosis (MS) lesions. Both in silico and in vitro studies show that MMP7 degrades glycosylated and basement membrane bound perlecan and plays a vital role in prostate cancer cell invasion.PMID 24833109

物理形态

10 mM HEPES、5 mM CaCl 2、150 mM NaCl溶液

分析说明

一个单位的酶活性定义为在pH 7.5和37 °C下消化1 μg azocol/min。

技术规格