Sigma-Aldrich Fas Ligand human，重组体，在CHO细胞中表达，≥95%（SDS-PAGE），≥95%（HPLC），适用于细胞培养

产品概述

Fas配体（FasL）是TNF-α的一个成员。肿瘤坏死因子（tumor necrosis factor，TNF）是一个表达于活化T细胞表面的超家族，它可以以可溶形式存在于循环中或以膜结合形式存在于细胞中。重组人可溶性Fas配体（recombinant human soluble Fas ligand，FasL）是一个分子量为17.9kDa的蛋白质，由FasL的TNF同源区组成，其N端有一个8个残基的His-末端，与FasL的TNF同源区相连。人和鼠sFasL在人和鼠细胞上都是完全活性的。

应用领域

Fas ligand human has been used to induce apoptosis in T-cell lymphoma-derived HuT78 cellsPMID 27929746 and jurkat cells.PMID 28439094

生化/生理作用

Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-Associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death.PMID 10075697 FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy.PMID 26479732 Changes in the activity of FasL suppresses normal apoptosis, leading to abnormal survival and growth of tumor cells.PMID 26617948 Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome.PMID 26690594

物理形态

冻干，无添加剂。

制备/使用说明

打开前将小瓶冲洗干净。用水复溶至浓度为0.1 - 1.0 mg/ml。不要涡旋。该溶液可在2 - 8 ° C下储存长达1周。为了延长储存时间，建议在含有载体蛋白（例如0.1% BSA）的缓冲液中进一步稀释，并在-20 °C至-80 °C下以工作等分试样储存。

其他说明

HHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL

技术规格