Sigma-Aldrich Peroxiredoxin II Active human，重组，在杆状病毒感染的昆虫细胞中表达，≥80%（SDS-PAGE）

产品概述

Research area: CELL SIGNALING

Peroxiredoxin 2 (PRDX2) is a typical peroxiredoxin, with 2-cysteine (Cys), a catalytic Cys51 and a resolving cysteine Cys172.PMID 21750082 Peroxiredoxins are peroxides reducing enzymes, and 2-Cys Prx subfamily contains four members in mammals, including PRDX1-4. PRDX2 is the predominant peroxidase present in mammalian erythrocytes, and its nitrated form is present in brains of patients with early Alzheimer’s disease.PMID 24719319

应用领域

Peroxiredoxin II Active human已被用作牛卵泡卵母细胞IVM（体外成熟）培养基的补充剂。

生化/生理作用

Peroxiredoxin-2 is a ubiquitous redox-active intracellular enzyme that acts as a redox-dependent inflammatory mediator, triggering macrophages to produce and release TNF-α (tumor necrosis factor). It may be involved in peroxidase activity and resistance to overoxidation.PMID 24719319 Its levels are elevated in several human cancer cells and tissues, including colorectal cancer (CRC) and it influences diverse cellular processes involving cells survival, proliferation and apoptosis. Prdx2 plays an essential role in regulating oxidation-induced apoptosis in CRC cells and may have potential as a therapeutic target in CRC.PMID 24234423 It may also act as a possible candidate biomarker for pancreatic cancer.PMID 24222118 Prx2 is a key regulator of invasion and metastasis in melanoma.PMID 23749642

物理形态

在25 mM Tris-HCl（pH 8.0）、100 mM NaCl、0.05%吐温-20、50%甘油和3 mM DTT中配制。

制备/使用说明

在冰上解冻。第一次解冻时，短暂旋转含酶的试管，以回收试管中的全部内容物。将酶等分为一次性使用的等分试样。将剩余的未稀释的酶等分试样储存在-70 °C下。注：酶对冻融循环非常敏感。

技术规格